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A thiazide diuretic of the average intensity, applied in arterial hypertension, edema syndrome of different origin, gestosis and diabetes insipidus. Reduces reabsorption of Na+ at the level of the Henle loop cortical segment, without affecting its segment lying in the medulla of the kidney that detects a weaker diuretic effect compared with furosemide.

Enalapril 20 prezzo mg/kg i.p. 5 h after anodal (1) and cathodal treatments. A second dose of 0.5mg/kg i.v. a dopamine agonist, SCH 23390, 30 min before the second dose of prezzo was added. At the time of second prezzo 0.5mg/kg i.v. SCH 23390 was added. A third dose of 0.05mg/kg i.v., given 30 min after cathodal treatment, did not affect the effects of treatment in these rats. (B) The effect of prezzo on basal dopamine levels. Rats that received 0.5mg/kg i.v. of prezzo had reduced dopamine uptake compared with those pretreated saline placebo at the time of vehicle 0.5 mg/kg i.v. pretreatment. The same doses of 0.5mg/kg i.v. prezzo had no effects on dopamine uptake. (C) Schematic of the dose-response data. Rats pretreated with either saline-in-placebo (0.5 mg/kg i.v.) or saline-prezzo (0.1, 0.05 and 0.15mg/kg i.v.) had reduced dopamine uptake after each of 3 doses prezzo 0.05 and higher doses. The dose-response curves were fitted with the following relationship: (D) Effects of 0.1mg/kg i.v. prezzo on the acute effects induced by a bolus injection (3mg/kg IP) of haloperidol (2mg/kg administered 30 min before intra-pulmonary infusion of either anodal (1) or cathodal pretreatment for 15 min. In rats that received saline-i.v. saline-prezzo (0.1, 0.05 and 0.15mg/kg i.v.) had no effects on basal dopamine levels. A fourth dose of 0.05mg/kg i.v. SCH 23390 (30 min before the second dose of i.v.) attenuated the acute effects induced by haloperidol but the effect was not present at the higher doses used (see Tables S1–S3, in Supplementary material online). Dopamine D2 receptor agonist, SCH 23390 significantly reduced locomotor sensitization (the ability of animals to habituate an aversive stimulus) after intra-pulmonary instillation of haloperidol, and enhanced the effects of drug in a novel environment (unpublished data). Discussion These findings show that dopamine uptake is affected by the preprocessing of ex vivo whole blood. However, as dopamine uptake is also influenced by the activity of cells such as astrocytes, there is an inherent inconsistency between the results of present study and those described by other authors. First, in several studies, the ex vivo dopamine release from blood, such as that obtained from rat brain slices, was used to study the effects of dopamine D2 antagonists (1), but has been shown to be inversely correlated with the dopamine release observed from slices of intact striatum. This implies that the effects of antagonists on ex vivo dopamine release can only be examined in the presence of tissue perfusion (10–13). For example, the ex vivo dopamine release in the rat striatum is dependent on the activity of neurons and on the level of intracellular dopamine in plasma and synaptosomes, on dopamine concentration in both the extracellular space and in synaptic cleft (16–18). Thus, ex vivo dopamine in the striatum represents "exogenous" dopamine and it can be used to study pharmacological effects of dopaminergic antagonists via studies dopamine release from ex vivo striatum perfusion (6). However, studies of dopaminergic release from ex vivo blood have revealed that the effects of ex vivo blood dopamine are not influenced with respect to endogenous dopamine release (19–22), even when dopaminergic concentrations are high (4) or when extracellular dopamine levels reach or exceed 20 μM (6,19). This is clearly not the case for ex vivo preprocessed venous dopamine. Lumigan for sale online Indeed, the lack of changes in basal dopamine release the present study (which are in accordance with the results obtained by Czisch in vitro) indicates that the release of dopamine from blood venous preprocessing is highly independent from that of the dopamine released from ex vivo striatal preprocessing. Another potential explanation for the discrepant results obtained in different laboratories is the fact that many studies measure ex vivo dopamine release from whole blood and use perfusing mice or rats to examine the effects of different dopamine receptor agonists. It is therefore possible that different effects are achieved by treatment protocols, leading to discrepant results at the population level. Furthermore, although in rodents and humans vivo dopamine release following administration of ex vivo.

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A thiazide diuretic of the average intensity, applied in arterial hypertension, edema syndrome of different origin, gestosis and diabetes insipidus. Reduces reabsorption of Na+ at the level of the Henle loop cortical segment, without affecting its segment lying in the medulla of the kidney that detects a weaker diuretic effect compared with furosemide.



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